Roland Cy-5 Dual Trigger Cymbal Pad, 10In

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In solution, gently shake to mix, then centrifuge briefly to collect the sample at the bottom of the reaction tube. Don'tmix well to prevent protein samples from denaturation and inactivation. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or

Agbavwe, C. & Somoza, M. M. Sequence-dependent fluorescence of cyanine dyes on microarrays. PLoS ONE 6, e22177 (2011). Though originally tested onboard Luda class missile destroyers and Jiangwei class missile frigates, the CY-1 can be carried by any surface combatant with C-801/802/803 launchers, from which the CY-1 can be launched, thus increasing the versatility and reducing the cost. In addition, a version is further modified so that it can be launched from torpedo tubes of submarines like the C-801, but there is not any confirmation that this version has entered the service. In an effort to boost possible export, the CY-1 has also been modified to carrying a various range of light torpedoes, such as that of USA, Italy, and Russian. However, there is no known export as of 2007. The CY-1 is also known to have been tested on the Type 039 submarine. The Cy3 and Cy5 nomenclature was first proposed by Ernst, et al. [5] in 1989, and is non-standard since it gives no hint of their chemical structures. In the original paper the number designated the count of the methines (as shown), and the side chains were unspecified. Due to this ambiguity various structures are designated Cy3 and Cy5 in the literature. The R groups do not have to be identical. In the dyes as used they are short aliphatic chains one or both of which ends in a highly reactive moiety such as N-hydroxysuccinimide or maleimide. structure or technology of the Products, or use the Products for the purpose of developing any products or services that would

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Depends strongly on viscosity, temperature, and biomolecular interactions. [11] Common cyanine dyes and their uses [ edit ] Sale, lease, license or other transfer of the material or any material derived or produced from it.

The protein must be in the buffer without primary amine (such as Tris or glycine) and ammonium ion, otherwise the labeling efficiency will be affected. Perez-Gonzalez, C., Lafontaine, D.A. & Penedo, J.C. Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes. Front. Chem. 4 (2016).CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.

Norman, D. G., Grainger, R. J., Uhrin, D. & Lilley, D. M. J. Location of cyanine-3 on double-stranded DNA: Importance for fluorescence resonance energy transfer studies. Biochemistry 39, 6317–6324 (2000). Cyanines were first synthesized over a century ago. They were originally used, and still are, to increase the sensitivity range of photographic emulsions, i.e., to increase the range of wavelengths which will form an image on the film, making the film panchromatic. [4] Cyanines are also used in CD-R and DVD-R media. The ones used are mostly green or light blue colour, and are chemically unstable. For that reason, unstabilized cyanine discs are unsuitable for archival CD and DVD use. Recent cyanine discs contain stabilizers, typically a metal atom bonded to the cyanine molecule, [6] that slow the deterioration significantly. These discs are often rated with an archival life of 75 years or more. The other dyes used in CD-Rs are phthalocyanine and azo. While patent protection for the standard Cy series of dyes has lapsed, the trademarked Cy naming remains in place. Consequently, dyes that are identical to Cy dyes, but called different names, are now sold. Sulfo–Cyanine dyes bear one or two sulfo groups, rendering the Cy dye water-soluble, but tri- and quadri-sulfonated forms are available for even higher water solubility. [8] PEGylation is another modification that confers hydrophilicity, not only to the dye but also to the labeled conjugate. Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: Flow cytometry, Microscopy (mainly Visible range, but also UV, IR), Microplate assays, Microarrays, as well as "light-up Probes," and in vivo imaging. [18] Nucleic acid labeling [ edit ]

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CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646. The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment. Kaur, G., Lewis, J.S. & van Oijen, A.M. Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA. Molecules 24 (2019). Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J. & Waggoner, A. S. Cyanine dye labeling reagents—sulfoindocyanine Succinimidyl Esters. Bioconjugate Chem. 4, 105–111 (1993).

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed Ranasinghe, R. T. & Brown, T. Ultrasensitive fluorescence-based methods for nucleic acid detection: Towards amplification-free genetic analysis. Chem. Commun. 47, 3717–3735 (2011).

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Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye. Harvey, B. J. & Levitus, M. Nucleobase-specific enhancement of Cy3 fluorescence. J. Fluoresc. 19, 443–448 (2009). Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Levitus, M. & Ranjit, S. Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments. Q Rev. Biophys. 44, 123–151 (2011). CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva.